Ine inenga-iti-rahi

Whakataki Hua

He momo taputapu tere, tika hoki te kimi i te kukū o te waikawa karihi, te pūmua me te otinga pūtau me te kore e whakamahanahia, ko te rahi o te tauira he 0.5 ki te 2ul anake, ā, ka taea e te aratau cuvette te kimi i te kukū o ngā huakita me ētahi atu momo ahurea. Ka taea te hono i te mahi kimi ira fluorescence me te kete tātari ine fluorescence, mā te whakakotahitanga motuhake o ngā tae fluorescent me ngā matū ūnga ka taea te ine tika i ngā kukū DNA, RNA me te pūmua, ā, ko te iti rawa ka taea te eke ki te 0.5pg/μl (dsDNA).

Ngā āhuatanga o te hua

He poto te auau o te kimo o te pūtake mārama, ki te whakaritea ki te tikanga kimi tuku iho, ka roa ake te ora o te pūtake mārama. Ka tere ake te kimi i te whakaohooho kaha o te mārama o ngā hua whakamātautau iti, ā, kāore e ngāwari te pirau.;

Mahi fluorescence: Mā te whakamahi i te matū fluorescence, ka taea te kite i te kukū pg dsDNA;

4 hangarau kimi ara whatu: hangarau whakahaere motuka ahurei, te whakamahi i te aratau kimi ara whatu "4", te pumau, te auau, te pai ake o te raina, he nui ake te whānuitanga ine;

Pūreretā ā-roto: Mā ngā kōwhiringa raraunga-ki-pūreretā ngāwari-ki-te-whakamahi, ka taea e koe te tā tika i ngā pūrongo mai i te pūreretā ā-roto.r;

OD600 otinga huakita, kimi moroiti: mā te pūnaha kimi ara whatu OD600, he watea te aratau rihi mō ngā huakita, ngā moroiti me ētahi atu kitenga kukū otinga ahurea.;

Teitei o te whakahokinga me te raina;