Ko te Kete Purehanga DNA a MagPure Bacteria Genomic DNA

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I muri i te rongoā ki te wai nakunaku, ka tukuna te waikawa karihi i roto i te tauira huakita mā te whakamahi i te Lysis Buffer anake. Ko te DNA ira kua tukuna ka herea motuhake ki ngā poroporo Aukume. Ko te DNA ira e herea ana ki ngā matūriki aukume ka hopukina e te rauemi aukume; ka tangohia ngā poke mā te horoi ki te Wash Buffer. Kātahi ka tangohia te waikawa karihi mai i ngā matūriki mā te whakamahi i te Elution Buffer. E tika ana mō ngā momo huakita karamu-kino katoa me ngā huakita pai, me ētahi atu.

WHAKAPĀ MAI

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Wahanga matua

Ngeru.#	BFMP10R1	BFMP10R16	BFMP10R	Cngā wāhanga
Pate cking	1T40T/kete	16T16T/kete	32T32T/kete
Pūmua K	400μ L x 2	320μ L	640μ L	Otinga protease (Kua tākai motuhake)
RNaseA	80μ L	32μ L	64μ L	Otinga whākōkī (Kua tākai motuhake)
Pūwero BA	12mL	5ml	10ml	Otinga tote
Pūwero BL	I roto i te whā tekau, ono-puna whakarite kātere 40 ngā wahi	96 puna kua tākaihia pereti 2 ngā wahi	96 puna kua tākaihia pereti 2 ngā wahi	Te denaturant kaha me te Tris buffer
Pūrere WA				Otinga tote
Pūnaha WB				Wairewa tote iti
Pūwero DE				Otinga Tris
Ngā poroporo aukume				Otinga poroporo aukume hauwai
Pukapuka Kaiwhakamahi	1	1	1