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Ka taea te wehewehe i ngā kiko kararehe ki ngā kiko epithelial, ngā kiko honohono, ngā kiko uaua me ngā kiko io i runga i ō rātou takenga mai, te āhua, te hanganga me ngā āhuatanga mahi noa, e hono tahi ana, e whakawhirinaki ana tetahi ki tetahi i roto i ngā ōwehenga rerekē hei hanga i ngā momo okana me ngā pūnaha o ngā kararehe hei whakaoti i ngā momo mahi ā-tinana.
Te kiko epithelial: he mea tito mai i ngā pūtau epithelial maha e tata ana te whakarite me te iti o ngā pūtau interstitial he rite te hanganga ki te kiriuhi, e kapi ana i te mata o te tinana kararehe me te tinana o ngā ngongo, ngā poka, ngā kāpū me te mata o roto o ētahi okana. Ko ngā mahi a te kiko epithelial he tiaki, he huna, he tuku me te mimiti.
Kiko hono: He mea tito mai i ngā pūtau me te nui o te matrix i waenga i ngā pūtau. Ko te kiko hono i hangaia e te mesoderm te momo kiko kararehe tino horapa me te kanorau, tae atu ki te kiko hono wetewete, te kiko hono kikī, te kiko hono reticular, te kiko cartilage, te kiko wheua, te kiko ngako me ērā atu. He mahi tautoko, he hononga, he tiaki, he parepare, he whakatikatika me te kawe.
Te kiko uaua: he mea tito mai i ngā pūtau uaua e āhei ana ki te whakawhāiti. He angiangi te āhua o ngā pūtau uaua pērā i te muka, nō reira e kiia ana hoki ko te muka uaua. Ko te mahi matua o te muka uaua ko te whakawhāiti me te hanga nekehanga uaua. E ai ki te āhua me te hanganga o ngā pūtau uaua me ngā mahi rerekē, ka taea te wehewehe i te kiko uaua ki te uaua angaanga (uaua whakawhiti), te uaua maeneene me te uaua ngākau.
Kiko io: he kiko e titoa ana i ngā pūtau io me ngā pūtau glial. Ko ngā pūtau io ngā waeine āhua me ngā waeine mahi o te pūnaha io, ā, he kaha ki te rongo i ngā whakaohooho ā-roto, ā-waho hoki, me te kawe i ngā akiaki i roto i te rauropi.
Hua Ika Nui
He pūnaha ārai motuhake tēnei e whakamahia ana e te hua, he mea hanga motuhake, he mea arotau hoki, ā, he mea here motuhake ki te DNA, ka taea te here tere me te mimiti, te wehe me te pure i ngā waikawa karihi. He pai mō te tango me te purenga whai hua o te DNA ira mai i ngā momo kiko kararehe me ngā whekau o roto (tae atu ki ngā rauropi moana), ā, ka taea te tango i ngā momo pūmua, ngako me ētahi atu matū waro me ētahi atu paru katoa. Ka taea te whakamahi me teIka nuiKo te Kaitango Waikawa Matū Aukume, he mea tino pai mō te tango aunoa i ngā tauira rahi. He tino parakore, he kounga teitei hoki ngā hua waikawa matū kua tangohia, ā, ka taea te whakamahi whānui i roto i te PCR/qPCR o raro iho, te NGS, te whakarōpūtanga Southern me ētahi atu rangahau whakamātautau.
Ngā Āhuatanga:
He whānuitanga o ngā tauira: ka taea te tango tika i te DNA ira mai i ngā momo tauira kiko kararehe katoa
Haumaru, kāore hoki e paitini: kāore he whakarewa paitini pēnei i te phenol, chloroform, me ētahi atu i roto i te matū, he nui hoki te tauwehenga haumaru.
Aunoatanga: ka taea te whakamahi i te taurite ki te Bigfish Nucleic Acid Extractor mō te tango tere-rahi, he mea tino pai mō te tango tauira nui
Teitei te parakore: ka taea te whakamahi mō ngā whakamātautau koiora ngota, pērā i te PCR, te nakunaku whākōkī me te whakaranu tika
Ngā Taputapu e Pā Ana: BFEX-32/BFEX-32E/BFEX-96E
Te tukanga tango:
Te whakamātautau: 25-30mg o te kiko kararehe
Te huri: te huri hauota wai, te huri, te kutikuti rānei i te mīhini huri
Nakunaku: 56℃ nakunaku kaukau mahana
Te whakaurunga: te whakamahi i te pūwero hei tango i te wai i runga, ka tāpirihia ki te pereti poka hohonu hei tango i runga i te papa.
Ngā raraunga whakamātautau: I tangohia he 30mg o ngā tauira kiko mai i ngā wāhanga rerekē o ngā kiore, ā, i mahia te tango me te purenga DNA ki te BFMP01R e ai ki ngā tohutohu. I whakaatuhia e ngā hua whakamātautau he pai te tere tango o te kete BFMP01R.
Wā tuku: Hurae-17-2025
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