2× SYBR kakariki qPCR MIX（Me te ROX Teitei）

Ngā āhuatanga o te hua

Ko tēnei hua, 2×SYBR green qPCR MIX, kei roto i te ngongo kotahi kei roto ngā wāhanga katoa e hiahiatia ana mō te whakanui me te kimi i te PCR, tae atu ki te Taq DNA poLymerase, te tae SYBR green I, te Tae Tohutoro ROX Teitei, ngā dNTP, Mg2+, me te pūpare PCR.
He tae kanapa matomato te tae kakariki I SYBR e herea ana ki te rohe rua-whiri DNA (DNA aho-rua, dsDNA) o te rua helix iti. Ka kanapa ngoikore te SYBR matomato I i te āhua kore utu, engari ina herea ki te DNA aho-rua, ka tino whakanuia tōna kanapa. Mā tēnei ka taea te ine i te nui o te DNA aho-rua i hangaia i te wā whakanui PCR mā te kite i te kaha kanapa.
Ka whakamahia te ROX hei tae whakatikatika hei whakatika i ngā rerekētanga o te hihi-ā-rangi kāore e pā ana ki te PCR, ā, ka whakaitihia ngā rerekētanga ā-wāhi. Ka taea te whakaputa i ēnei rerekētanga e ngā āhuatanga maha, pērā i te hapa o te pipette, te mimiti rānei o te tauira. He rerekē ngā hiahia o ngā taputapu ine hihi-ā-rangi mō te ROX, ā, he pai tēnei hua mō ngā tātari ine hihi-ā-rangi e hiahia ana ki te whakatikatika ROX Teitei.