He aha te tikanga mō te wehe me te pure i ngā waikawa karihi?

Ngā waikawa karihi, tae atu ki te DNA me te RNA, he koiora nui e whai wāhi nui ana ki te ira, te koiora ngota, me te hangarau koiora. He mea nui te kaha ki te wehe me te pure i ēnei waikawa karihi mō ngā momo tono, tae atu ki te tāruarua, te raupapatanga, me te tātari whakaaturanga ira. Kei roto i ngā pūnaha purenga waikawa karihi he whānuitanga o ngā tikanga i hangaia hei tango me te pure i ngā waikawa karihi mai i ngā tauira koiora. Ka tūhuratia e tēnei tuhinga ngā tikanga mō te wehe me te pure i ngā waikawa karihi, ā, ka whakamāramahia te hiranga o ēnei pūnaha i roto i te rangahau pūtaiao hou.

Te mārama ki te purenga waikawa karihi

Ko te purenga waikawa karihi e pā ana ki te tango i te DNA, i te RNA rānei mai i ngā pūtau, i ngā kiko rānei, ā, muri iho ka tangohia ngā poke pērā i ngā pūmua, ngā lipid, me ētahi atu para pūtau. He mea nui te parakore me te pono o ngā waikawa karihi kua wehea mō ngā tono o muri mai, nā te mea ka taea e ngā poke te aukati i ngā tauhohenga whākōkī me te pā ki te tika o ngā hua whakamātautau.

Ngā tikanga noa mō te wehewehe me te purenga o te waikawa karihi

Te tango i te phenol-chloroform:Ka whakamahia e tēnei tikanga tuku iho ngā whakarewa rauropi hei wehe i ngā waikawa karihi mai i ngā pūmua me ētahi atu wāhanga pūtau. Ka konatunatua te tauira ki te phenol me te chloroform, ka wehea ngā waikawa karihi ki te wāhanga wai, engari ka noho tonu ngā pūmua ki te wāhanga rauropi. I muri i te centrifugation, ka kohia te wāhanga wai kei roto ngā waikawa karihi, ā, ka whakapūpūtia ki te etanoru.

Ngā tikanga e hangai ana ki te kiripaka:He whānuitia te whakamahinga o ngā kiriuhi hāriki i roto i ngā kete purenga waikawa karihi arumoni. Ko te kaupapa o tēnei tikanga ko te herea o ngā waikawa karihi ki te hāriki hāriki i ngā kukū tote teitei. Whai muri i te herea, ka horoia atu ngā poke, kātahi ka horoia ngā waikawa karihi ki te wai, ki te wai rānei, he iti te tote. He pai tēnei tikanga nā te mea he tere, he whai hua, ā, he tino parakore ngā waikawa karihi.

Te purenga o te pīrere aukume:Ka whakamahia e tēnei tikanga ngā pirepire aukume kua pania ki ngā matū here waikawa karihi. Ina konatunatua he tauira ki ngā pirepire aukume, ka piri ngā waikawa karihi ki te mata o ngā pirepire. Kātahi ka wehea ngā pirepire mai i te otinga mā te whakamahi i te aukume, ā, ka tangohia ngā poke. He maha ngā whakamahinga o tēnei tikanga, ā, ka taea te whakahaere aunoa, kia pai ai mō ngā tono tere-whakaputa.

Te chromatography pou:Ko tēnei tikanga ko te tuku i tētahi tauira mā roto i tētahi pou chromatography kua whakakiia ki tētahi wāhanga tūmau e pupuri ana i ngā waikawa karihi. Ka taea te whakamahi i ngā momo pou rerekē, tae atu ki ērā e hangai ana ki te tango rahi, ki ngā mātāpono whakawhiti katote rānei. Ka puta ngā waikawa karihi mai i te pou, ka hua ake he tauira kua purea.

Ngā tikanga whākōkī:Ka taea te whakamahi i ngā tikanga whākōkī, pērā i ērā e whakamahi ana i te DNase, i te RNase rānei, hei whakaiti i ngā waikawa karihi, i ngā poke rānei kāore e hiahiatia. He tino whai hua tēnei tikanga ina tukatukahia ngā tauira uaua, pērā i ērā kei roto ko te DNA me te RNA.

Hei whakamutunga

He mea nui te wehenga me te purenga o te waikawa karihi i roto i te rangahau me ngā tono o te koiora ngota.Ngā pūnaha purenga waikawa karihie tuku ana ki ngā kairangahau he momo tikanga hei whiwhi i ngā waikawa karihi kounga teitei e tika ana mō ngā tono o muri. Ahakoa te whakamahi i te tango phenol-chloroform tuku iho, ngā tikanga hou rānei pēnei i te silica gel, te purenga i runga i te piripiri aukume rānei, ko te whiriwhiri i te tikanga e whakawhirinaki ana ki ngā whakaritenga motuhake o te whakamātautau me te āhua o te tauira. I roto i ngā whanaketanga hangarau, kua whanake tonu ēnei pūnaha purenga, e whakapai ake ana i te pai, te tere, me te pono, ā, i te mutunga ka whakarei ake i ngā kaha o ngā kairangahau i roto i te mara o te koiora ngota.