He aha ngā raruraru noa o ngā Thermocyclers?

 

Ngā Take Taurite o te Mahana

Ko te raru nui rawa atu o te huringa wera ko ngā rerekētanga o te pāmahana puta noa i te poraka. Ka puta he hua whakanui rerekē nā te wera kore taurite, ka whakararu i te pono o te whakamātautau. KoungaKaihurihuri Wera FastCyclerKa whakamahia e ngā tauira ngā huānga Peltier matatau me ngā rauropi matatini hei pupuri i te ōritetanga ±0.2°C puta noa i ngā puna katoa. Heoi anō, mā te tawhito o ngā poraka whakamahana, te whakapiri wera kua pakaru, ngā paru kua kohia rānei ka heke te mahi i roto i te wā.

Ngā tohu o ngā raruraru pāmahanaKo te korenga o ngā tauhohenga PCR i ngā tūranga poka motuhake, ngā pihi rewa kāore i te ōrite, ngā hua hua rerekē rānei puta noa i te pereti tauira kotahi e tohu ana i ngā take ōritetanga e hiahiatia ana te whakatikatika tonu.

Ngā hapa o te whakamahana o te taupoki

Ka ārai ngā taupoki wera i te haukū e whakapōrearea ana i ngā ranunga tauhohenga, ā, ka whakaiti i te whai huatanga o te PCR. Ko ngā rahunga whakamahana taupoki tētahi o ngā amuamu tino maha mō ngā mīhini huringa wera. Mā te kore e ranea te pāmahana taupoki ka taea te hanga haukū, engari mā te wera nui ka taea te whakakore i te āhua o ngā tauira, te kino rānei i ngā mea kirihou e pau ana.

Kei ngā pūnaha FastCycler Thermal Cycler hou te whakamahana taupoki ka taea te whakarite (100-110°C te tikanga) me te whakahaere pāmahana tika. Kei roto i te tiaki auau te tirotiro i ngā tikanga pēhanga taupoki me ngā pūoko pāmahana kia tika ai te whakapā me te tohatoha wera.

Te hekenga o te tere piki

Mā te tere o te pikinga ka rerekē ngā mīhini hikohiko kounga teitei mai i ngā tauira taketake. I roto i te wā, ka puhoi pea ngā tere whakamahana me te whakamatao nā te pakaru o te huānga Peltier, te pakaru o te pā hau, ngā raruraru rānei o te hauhautanga i roto i ngā pūnaha whakamatao. Ka roa ake te huringa o ngā huringa nā tēnei pirau, ā, ka pā ki ngā tauhohenga tairongo ki te pāmahana.

Ka mau tonu te tere o te pikinga (4-5°C/hēkona) o ngā taputapu FastCycler Thermal Cycler kounga ngaio mā roto i ngā huinga Peltier takirua me te whakahaere wera kua arotauhia. I te hoko, me manatoko ngā whakaritenga whakamahana me te whakamatao, kaua ko ngā tere pikinga mōrahi anake.

Ngā Raru Pūmanawa me te Hononga

Ka whakauruhia e ngā miihini whakamahana hou ngā pūmanawa uaua mō te hōtaka kawa, te takiuru raraunga, me te hononga whatunga. Ko ngā take pūmanawa noa ko:

Ngā hapa pūmanawa: Te take o ngā tukinga o te hōtaka, te hē rānei o ngā pānui pāmahana

Ngā hapa o te USB/EthernetTe aukati i te whakawhiti raraunga, te aroturukitanga mamao rānei

Ngā hapa o te mata pa: Te whakauaua i te hōtaka kawa

Ngā take hototahiMe ngā pūnaha whakahaere mōhiohio taiwhanga (LIMS)

Ka whakaratohia e ngā kaihanga matua ngā whakahōu pūmanawa me te tautoko hangarau urupare hei whakatika wawe i ēnei wero.

Te Pakaru me te Roimata Mīhini

Ka heke haere te kounga o ngā wāhanga ā-tinana:

Parahanga poraka: Ka puta he pānga wera kore ōrite i ngā tauira i maringi, ā, me horoi pai.

Te pakaru o te hononga o te taupoki: Ka ngoikore ngā wāhanga miihini i te whakatuwheratanga pinepine

Ngā rahunga o te pāTe whakaiti i te pai o te whakamatao me te whakaroa i ngā wā huringa

Te nekenekehanga o te pūoko: Te whakaputa i ngā pānui pāmahana hē e hiahia ana kia whakatikatika anō

Te Hurihanga Whakatikatika

Me manatoko i ngā whakatikatika ā-wā mō ngā huringa wera katoa. Ka nekeneke noa ngā pūoko pāmahana i roto i te wā, ka puta pea he hapa nui i roto i ngā whakamātautau. Me whakahaere e ngā taiwhanga ngaio ngā tirotiro whakatikatika ā-hauwhā mā te whakamahi i ngā inemahana tohutoro kua whakamanahia.

KoungaKaihurihuri Wera FastCyclerKei roto i ngā tauira ngā āhuatanga tātaritanga-ā-roto e whakatūpato ana i ngā kaiwhakamahi ki ngā hiahia whakatikatika i mua i te pānga o ngā raruraru ki ngā hua. Kei ētahi pūnaha matatau ngā kawa whakatikatika aunoa e whakaiti ana i te wawaotanga ā-ringa.

Ngā Rautaki Tiaki Ārai

Whakaitihia ngā raruraru o te thermocycler mā te tiaki matatau:

• Horoia ngā poraka whakamahana i ia marama mā te whakamahi i ngā whakarewa e tika ana
• Manatokohia te tika o te pāmahana i ia toru marama mā te whakamahi i ngā pūwero kua whakatikatikaina
• Whakahōuhia te pūmanawa i ia wā kia uru atu ai ki ngā whakapainga me ngā whakatikatika pepeke
• Whakakapia ngā mea e pau ana (ngā hiri taupoki, ngā papa whakamahana) e ai ki ngā whakaritenga a te kaihanga.
• Kia mau tonu te hauhautanga huri noa i ngā taputapu kia pai ai te whakamatao

Te Kōwhiri i ngā Taputapu Pono

I te wā e hoko ana i ngā mīhini whakamahana, me whakarite kia rite ki ngā kaihanga e tuku ana i:

Ngā pūtāhui whānui: Te hipoki i ngā wāhanga e rua me te mahi

Tautoko hangarau urupareMe te tere o te wātea o ngā wāhanga whakakapinga

He rekoata whai hua kua whakamātauhiaTe pono kua whakaaturia i roto i ngā taiwhanga hoa

Te tiaki ngāwari ki te whakamahiNgā wāhanga e wātea ana, me ngā tuhinga ratonga mārama

Whakamutunga

Ahakoa he maha ngā wero whakahaere ka puta mai i ngā mīhini whakamahana hiko, mā te mārama ki ngā raruraru noa ka taea te whiriwhiri taputapu whai whakaaro me te whakamahere tiaki tika. Ka whakaitihia te wā kore mahi a te haumi ki ngā pūnaha FastCycler Thermal Cycler kounga teitei me te hanganga tautoko pakari, ā, ka whakarite kia rite, kia pono hoki ngā hua PCR. Aromatawaihia te utu katoa o te mana whakahaere—tae atu ki ngā whakaritenga tiaki me te kounga tautoko—kaua ko te utu hoko anake. Ka noho te mīhini whakamahana hiko tika hei hoa taiwhanga pono e tuku ana i ngā tau maha o te mahi kore raruraru me ngā putanga pūtaiao ka taea te whakaputa anō.