Kete Whakamaarama DNA o te Bigfish FeiXu: He Otinga Tino Whai Hua mō te Tangohanga DNA Tauira-ā-Waha

I ngā mara ote tātaritanga haumanu in vitro (IVD), te ira-ira, me te rangahau ngota, ngā tauira ā-waha—pērā ingā ūkui waha, ngā ūkui korokoro, me te huware—e whakamahia whānuitia ana mō te whakamātautau waikawa karihi nā te meahe ngāwari te kohikohi, kāore e pokanoa, ā, kāore he mamae o te tukanga tauiraHeoi anō, ko ngā tauira ā-waha kei roto i te nuinga o te wāhe iti noa ngā rahinga o ngā waikawa karihiā, he maha ngā wā kua poke i tengā pūmua me ētahi atu pokeHe maha ngā wā ka pāngia ngā tikanga tango tuku iho e tengā rerenga mahi uaua, te iti o te whai huatanga, me te whakamahinga o ngā matū paitini, ka taea te whakararu nui i te tika me te pumau o ngā tono o muri mai pēnei i tePCR/qPCR me te raupapatanga o te whakatupuranga hou (NGS).

TeKete Tango Ira Ira BFMP06 mō te Ārai Waha e Hangai Ana ki te Pīni Aukume, i whakawhanakehia eTe Hangarau Koiora FeiXu o Hangzhou Bigfish, e tuku ana i tētahiotinga haumaru, whai hua, me te ponomō te tango i te DNA tauira ā-waha. Nā tōna hoahoa hangarau auaha me ngā paerewa mahi pakari, kua noho tēnei kete hei taputapu pono mō ngā taiwhanga haumanu me ngā taiwhanga rangahau.

Kua hangaia te kete BFMP06 huri noa i tepūnaha parenga tino arotaui honoa kiNgā poroporo aukume hydroxyl motuhake-DNA, e hanga ana i tētahi rerenga mahi purenga waikawa karihi tino whai hua. I muri i te pakaru o te tauira i roto i te pūhui pakaru, ka pakaru ngā wāhanga pūtau, ā, ka tukuna ngā waikawa karihi. Ka herea e ngā rōpū mahi i runga i te mata o ngā pirepire aukume te DNA kore utu, ka hanga he pumaungā matatini pūmua-DNA.

I raro i tētahi papa aukume o waho, ka pāngia ngā matatini ee rua ngā taahiraa horoi tikakia tino tangohia ngā pūmua, ngā tote, me ētahi atu poke. Hei whakamutunga,DNA ira tino parakoreka tātarihia pai mā te whakamahi i te pūpare tātari.

Whakaahuatanga Hua