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Ko te raihi tētahi o ngā hua matua tino nui, nō roto i ngā tipu otaota wai o te whānau Poaceae. Ko Haina tētahi o ngā kāinga taketake o te raihi, e whakatipuhia whānuitia ana i te tonga o Haina me te rohe ki te Rāwhiti-mā-rāwhiti. Nā te whanaketanga o te pūtaiao me te hangarau, kua whakamahia whānuitia ngā tikanga koiora ngota hou ki te rangahau raihi. Mā te whiwhi i te DNA ira raihi kounga teitei, parakore, ka whakatakoto i tētahi turanga pakari mō ngā rangahau ira o muri mai. Mā te BigFish Sequence Magnetic Bead-Based Rice Genomic DNA Purification Kit ka taea e ngā kairangahau raihi te tango i te DNA raihi mā te ngāwari, te tere, me te whai hua.
Kete Whakamaemae DNA Ira Raihi
Tirohanga Whānui mō te Hua:
Ka whakamahia e tēnei hua tētahi pūnaha ārai motuhake kua whakawhanakehia, kua arotauhia hoki, me ngā pīrere aukume me ngā āhuatanga here DNA motuhake. Ka tere te here, te mimiti, me te wehewehe i ngā waikawa karihi, me te tango pai i ngā paru pēnei i ngā polysaccharides me ngā matū polyphenolic mai i ngā tipu. He tino pai mō te tango i te DNA ira mai i ngā kiko rau tipu. Ka honoa ki te BigFish Magnetic Bead Nucleic Acid Extraction Instrument, he tino pai mō te tango aunoa i ngā rahinga tauira nui. Ko ngā hua waikawa karihi kua tangohia he tino parakore me te kounga pai, e whai hua whānuitia ana mō ngā rangahau whakamātautau o muri mai pēnei i te PCR/qPCR me te NGS.
Ngā Āhuatanga o te Hua:
Haumaru, kāore he paitini: Kāore e hiahiatia he matū paitini pēnei i te phenol/chloroform
Te tere tere aunoa: Ka honoa ki te mīhini tango waikawa karihi raupapatanga Beagle, ka taea e ia te mahi tango tere tere, ā, he pai mō te tango i ngā tauira rahi.
Teitei o te parakore me te kounga pai: He tino parakore te hua i tangohia, ā, ka taea te whakamahi mō te NGS o raro, te whakapūtanga maramara me ētahi atu whakamātautau.
Ngā taputapu hototahi: BigFish BFEX-32/BFEX-32E/BFEX-96E
Wā tuku: Hepetema-11-2025
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