Hua Hou a Bigfish - Te Kirimi Agarose Kua Whakarewahia ki te Mākete

Whakaahuatanga Poto:

Ngā roopu haumaru, tere, pai
Kei te wātea inaianei te agarose gel kua oti te hanga mō Bigfish
He tiēre agarose kua oti te whakarewa

WHAKAPĀ MAI

Taipitopito Hua

Ngā Tohu Hua

Whakataki Hua

Ko te tiēre agarose kua oti te hanga he momo pereti tiēre agarose kua oti te whakarite, ka taea te whakamahi tika i roto i ngā whakamātautau wehewehe me te purenga o ngā macromolecules koiora pērā i te DNA. Ki te whakatauritea ki te tikanga whakarite tiēre agarose tuku iho, he ngāwari te whakahaere, he penapena wā, he pai te pumau o te tiēre agarose kua oti te hanga, ka taea te whakapai ake i te whai huatanga o te whakamātautau, te whakaiti i te rerekētanga o te whakamātautau, ā, ka taea e ngā kairangahau te arotahi ake ki te whiwhi me te tātari i ngā hua whakamātautau.

Whakatakotoranga

Ko ngā hua tiēre agarose kua oti te hanga i mua nā Bigfish i whakamahi i te tae waikawa karihi GelRed kore-paitini, he mea e tika ana mō te wehewehe i ngā waikawa karihi mai i te 0.5 ki te 10kb te roa. Kāore he DNase, RNase me te Protease i roto i te tiēre, ā, he papatahi, he mārama, he ngawari hoki ngā whitiki waikawa karihi, ā, he tino taumira.